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Drivers have been warned to take care on the A66 in the run up to Cumbria's Appleby Horse Fair.
The week-long event is the largest horse fair in Europe attracting 30,000 people and more than 1,500 caravans and horse drawn vehicles.
Cumbria and Durham police forces have been working together to minimise accidents and problems on the roads before and during the event.
The annual gathering of travellers will be held between 3 and 9 June.
Last year, caravans and cars caused traffic problems when they parked in lay-bys and on grassed areas in Barnard Castle's Bridgegate.
A 300m (980ft) stretch will see lay-bys closed between Thorngate and the County Bridge from 24 May to 14 June.
Travellers will instead be encouraged to stop in specially-allocated locations in Broomielaw and Shaw Bank, both on the A688.
The restrictions follow consultation with Teesdale Residents' and Travellers' Forum and Durham County Council.
Despite the use of special warning signs and speed enforcement, tragically since June 2003 there have been two fatal and one serious injury collision involving horse drawn caravans on the A66 travelling to and from the Fair.
Steve Bishop, the A66 route manager for the Highways Agency said: "It is paramount that motorists take special care due to the presence of slow moving vehicles."